Journal: Advanced Science

Article Title: Tumor‐Associated Sympathetic Nerves Promote the Progression of Epstein‐Barr Virus‐Positive Diffuse Large B‐Cell Lymphoma

doi: 10.1002/advs.202413580

Figure Lengend Snippet: Establishment of Epstein‐Barr virus (EBV)‐infected diffuse large B‐cell lymphoma (DLBCL) cell lines. A) Schematic illustration showing the process of establishing EBV‐positive DLBCL cell lines. EBV‐negative DLBCL cell lines (SUDHL4 and SUDHL6) were infected with cell‐free EBV derived from Akata cells and subsequently selected using G418. This figure was created by Figdraw under copyright code‐[RURPI77371]. B) Quantification of latency gene expression in different cell lines by quantitative reverse transcription PCR (qRT‐PCR), including EBER1, EBER2, LMP1, LMP2A, EBNA1, EBNA2, EBNA3A, EBNA3B, and EBNA3C. Gene expression was normalized to β‐actin. C) Western blot analysis confirms the expression of EBNA2 and LMP1 in the indicated cell lines. Akata cells were stimulated with 0.8% goat anti‐human IgG for 6 h. D) Mapping analysis of transcript sequences from tumors derived from SUDHL6‐EBV or SUDHL6 cells against the Akata genome. E) Assessment of latency genes expression in xenograft tumors from EBV positive DLBCL cells and their parental counterparts. F) Representative images of EBERs‐ISH in tumor tissues from mice implanted with EBV‐positive DLBCL cells or their parental cells.

Article Snippet: The human diffuse large B‐cell lymphoma cell lines SUDHL4, SUDHL6, and Farage were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Virus, Infection, Derivative Assay, Gene Expression, Reverse Transcription, Quantitative RT-PCR, Western Blot, Expressing